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Flexible, Customizable Comprehensive Genomic Profiling
Get the solid tumor insights you need, when you need them with flexible panel content, the choice to add on genomic signatures as needed, and workflows that work for you. Have confidence in calls with demonstrated performance with Archer technology.
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Figure 1. Illustration of Archer’s flexible, customizable CGP solutions. Having the ability to pick the targets you need allows you to optimize sequencing resources. The ability to customize panel content means you can adjust your targets quickly, without having to change your established protocols.
Choose the insights you need, when you need them with comprehensive genomic profiling that flexes to your needs. You can combine pre-designed panels, add on targets, or create panel content from scratch. If needed on a by-sample basis, spike-in panel content can be used for only the samples that require it. Genomic signature modules for MSI and TMB can be added in for every sample or on a by-sample basis, allowing for optimal use of sequencing resources. If you need to add new biomarkers in the future, Archer CGP solutions enable you to easily add targets to your in-use assay, without compromising performance and with minimal workflow disruption.
Table 1. Efficiently generate a comprehensive genomic profile for solid tumor samples.
| Panels | Input | Total genes targeted | Reads | Genomic alterations detected | Samples* per NextSeq 500/550 high output kits v2.5 |
|---|---|---|---|---|---|
| FUSIONPlex™ Pan Solid Tumor v2 & VARIANTPlex™ Pan Solid Tumor | RNA & DNA | 279 | 28.5M | Fusions, SNVs, indels, CNVs, ITDs, expression, MSI, TMB | 14 |
| FUSIONPlex Pan Solid Tumor v2 & VARIANTPlex Complete Solid Tumor | RNA & DNA | 511 | 48.5M | Fusions, SNVs, indels, CNVs, ITDs, expression, MSI, TMB | 8 |
Data assumes Archer libraries are prepared using liquid adapters.
*Sample = 1 VARIANTPlex library + 1 FUSIONPlex library
Flexible CGP content means you use sequencing resources on insights you need without wasting sequencing reads on targets that aren’t needed. VARIANTPlex and FUSIONPlex panels can be paired for resource-efficient solutions to provide a customized CGP solution providing SNVs, indels, ITDs, fusions, and expression alterations as needed.
Workflows that work for you
Figure 2. Parallel workflows for VARIANTPlex and FUSIONPlex research assays. Similar library preparation protocols, pooled sequencing, and analysis allow for efficient genomic profiling of solid tumor samples.
Library preparation workflows can be run in parallel, while sample indexing allows for all Archer libraries to be sequenced together. Flexible run sizes are available with choice of lyophilized reagents in 8-tube strips, allowing for runs as small as one sample, or 96-well breakaway plates for larger runs. Scalable, high-throughput, liquid reagents are available in single-use 24- and 96-reaction kits. Regardless of format, Archer reagents are color-coded to match protocol steps for easier benchwork.
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Confidence in calls with demonstrated performance
Archer CGP solutions are powered by AMP™ chemistry and Archer Analysis, which have advantages for detection of novel and known fusions and sensitive variant identification in low-input samples over other NGS methods. Additionally, Archer solid tumor assays have demonstrated performance with:
MSI: The VARIANTPlex MSI Module enables accurate quantification of MSI status from FFPE-derived tumor samples, as determined by comparison with orthogonal methods and reproducibility studies.
TMB: VARIANTPlex Pan Solid Tumor and Complete Solid Tumor panels can provide measurement of TMB, with demonstrated high concordance with WES-based TMB. The innovative inclusion of a novel somatic/germline classifier powers the TMB calculation without a paired normal sample.
Customized panel content: Customization is enabled by AMP primers functioning independently, meaning gene content can be easily mixed in without compromising assay performance.
Figure 3. High performance regardless of primer pool size. Primer uniformity, coverage uniformity, and on-target percent are all ≥ 95% for small (n = 228 primers), medium (n = 554), and large (n = 2058) panels tested.
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